Hotspot

trnfM-CAU-trnT-GGU

LSC · 12997-15371 · 2375 bp

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Pi 0.0163
MarkerSeek score 56.10
Primer available yes
Label trnfM-CAU-trnT-GGU

Feature Metadata

label_name
trnfM-CAU-trnT-GGU
feature_type
igs
region
LSC
length_bp
2375
variable_sites
125
pi
0.0163
unique_haplotype_count
17
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnfM-CAU-trnT-GGU_p1 CCTCAAGGTTATGAGCCTCG CCACTGAGTTAAAAGGGC 55.0 50.0 57.8 52.9 2210-2474 bp 1.0 48.3
trnfM-CAU-trnT-GGU_p2 CCTCGTGAGCTACCAAACTG CCACTGAGTTAAAAGGGC 55.0 50.0 58.3 52.9 2195-2459 bp 1.0 48.3
trnfM-CAU-trnT-GGU_p3 CAAGGTTATGAGCCTCGTGA CCACTGAGTTAAAAGGGC 50.0 50.0 57.3 52.9 2207-2471 bp 1.0 48.3
trnfM-CAU-trnT-GGU_p4 TCAAGGTTATGAGCCTCGTG CCACTGAGTTAAAAGGGC 50.0 50.0 57.3 52.9 2208-2472 bp 1.0 48.3
trnfM-CAU-trnT-GGU_p5 TATGAGCCTCGTGAGCTACC CCACTGAGTTAAAAGGGC 55.0 50.0 58.7 52.9 2201-2465 bp 1.0 48.3