Hotspot

trnN-trnR

IRa · 128954-129569 · 616 bp

Back to Orychophragmus

Pi 0.004

MarkerSeek score 57.30

Primer available yes

Label trnN-trnR

Feature Metadata

label_name: trnN-trnR
feature_type: igs
region: IRa
length_bp: 616
variable_sites: 6
pi: 0.004
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-trnR_p1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	748-757 bp	1.0	79.9
trnN-trnR_p2	CCGTTGTTCCTCAGTAGCTC	GAACAGATCCAGTGGAGACG	55.0	55.0	58.0	57.7	785-794 bp	1.0	79.3
trnN-trnR_p3	TGGGTTGAGAACGGGAATTG	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.1	57.8	789-798 bp	1.0	79.1
trnN-trnR_p4	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	699-708 bp	1.0	78.8
trnN-trnR_p5	TGGGTTGAGAACGGGAATTG	GAACAGATCCAGTGGAGACG	50.0	55.0	58.1	57.7	826-835 bp	1.0	78.5