Hotspot

ndhC-trnV

LSC · 49728-50609 · 882 bp

Back to Orychophragmus

Pi 0.0112

MarkerSeek score 63.00

Primer available yes

Label ndhC-trnV

Feature Metadata

label_name: ndhC-trnV
feature_type: igs
region: LSC
length_bp: 882
variable_sites: 25
pi: 0.0112
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV_p1	TGCCAAAACAGGAATAGCACT	GGTTCGAATCCGTATAGCCC	42.9	55.0	58.1	57.9	776-978 bp	1.0	76.5
ndhC-trnV_p2	CCCCGGAAATTAGAAATGCC	GGTTCGAATCCGTATAGCCC	50.0	55.0	56.8	57.9	792-994 bp	1.0	76.1
ndhC-trnV_p3	TGCCAAAACAGGAATAGCACT	CAGGTCTACGGTTCGAATCC	42.9	55.0	58.1	57.8	785-987 bp	1.0	75.5
ndhC-trnV_p4	CCCCGGAAATTAGAAATGCC	CAGGTCTACGGTTCGAATCC	50.0	55.0	56.8	57.8	801-1003 bp	1.0	75.0
ndhC-trnV_p5	TGCCAAAACAGGAATAGCACT	CGGTTCGAATCCGTATAGCC	42.9	55.0	58.1	58.3	777-979 bp	1.0	74.7