Hotspot

trnS-trnG

LSC · 7872-8537 · 666 bp

Back to Orychophragmus

Pi 0.0206

MarkerSeek score 74.00

Primer available yes

Label trnS-trnG

Feature Metadata

label_name: trnS-trnG
feature_type: igs
region: LSC
length_bp: 666
variable_sites: 33
pi: 0.0206
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-trnG_p1	ACGCTTTAATCCACTCAGCC	AATCAAACCGAGAGACCCTT	50.0	45.0	58.3	56.5	761-788 bp	1.0	72.9
trnS-trnG_p2	ACGCTTTAATCCACTCAGCC	ATCAAACCGAGAGACCCTTT	50.0	45.0	58.3	56.5	760-787 bp	1.0	72.9
trnS-trnG_p3	CGCTTTAATCCACTCAGCCA	AATCAAACCGAGAGACCCTT	50.0	45.0	58.3	56.5	760-787 bp	1.0	72.9
trnS-trnG_p4	CGCTTTAATCCACTCAGCCA	ATCAAACCGAGAGACCCTTT	50.0	45.0	58.3	56.5	759-786 bp	1.0	72.9
trnS-trnG_p5	CGGAAGGGAAAGAGAGGGAT	ATCAAACCGAGAGACCCTTT	55.0	45.0	58.5	56.5	825-852 bp	1.0	70.6