Hotspot

ycf1

Genome · 97623-102446 · 4824 bp

Back to Orthotrichum

Pi 0.43

MarkerSeek score 74.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 4824
variable_sites: 1707
pi: 0.43
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACCAATGCACCTTTTGGAGA	ACGAATAGTTTGATGGCGCT	45.0	45.0	57.6	58.0	1503 bp	0.5	56.8
ycf1_p2	AGGCCTCTTTTTGGAAACCA	ACGAATAGTTTGATGGCGCT	45.0	45.0	57.5	58.0	1472 bp	0.5	56.0
ycf1_p3	ACCAATGCACCTTTTGGAGA	TTTGATGGCGCTCTCTTTCA	45.0	45.0	57.6	57.8	1495 bp	0.5	55.5
ycf1_p4	ACCAATGCACCTTTTGGAGA	AGTTTGATGGCGCTCTCTTT	45.0	45.0	57.6	57.8	1497 bp	0.5	55.4
ycf1_p5	AGGCCTCTTTTTGGAAACCA	TTTGATGGCGCTCTCTTTCA	45.0	45.0	57.5	57.8	1464 bp	0.5	54.8