Hotspot

rpl21

Genome · 110642-110992 · 351 bp

Back to Orthotrichum

Pi 0.491

MarkerSeek score 77.30

Primer available yes

Label rpl21

Feature Metadata

label_name: rpl21
feature_type: gene
region: Genome
length_bp: 351
variable_sites: 166
pi: 0.491
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl21_p1	GTGATGGAGATCGTGGGTTC	ACAAGAACTCCTACCGTAGT	55.0	45.0	58.1	55.2	1637 bp	0.5	37.3
rpl21_p2	ATTTCACCATGGCGGCTTAT	ACAAGAACTCCTACCGTAGT	45.0	45.0	57.9	55.2	1804 bp	0.5	37.2
rpl21_p3	ATAGCGTAGTTTGGCAACGT	ACAAGAACTCCTACCGTAGT	45.0	45.0	57.9	55.2	1668 bp	0.5	37.1
rpl21_p4	TCCTAAGAGCAGCGTGTCTA	ACAAGAACTCCTACCGTAGT	50.0	45.0	57.9	55.2	1827 bp	0.5	36.8
rpl21_p5	GGGGTATAGCGTAGTTTGGC	ACAAGAACTCCTACCGTAGT	55.0	45.0	58.1	55.2	1673 bp	0.5	36.8