Hotspot

ndhI

Genome · 106020-106571 · 552 bp

Back to Orthotrichum

Pi 0.434

MarkerSeek score 81.30

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: Genome
length_bp: 552
variable_sites: 231
pi: 0.434
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	GGGGATGGAATTTATGGCGT	AGCGCCTATTAATGCAACCA	50.0	45.0	58.0	57.9	1746 bp	0.5	59.6
ndhI_p2	TGGGGATGGAATTTATGGCG	AGCGCCTATTAATGCAACCA	50.0	45.0	58.0	57.9	1747 bp	0.5	59.5
ndhI_p3	GGGGATGGAATTTATGGCGT	AAAATTTGTGCGGCAGCTAC	50.0	45.0	58.0	57.9	1427 bp	0.5	59.4
ndhI_p4	TGGGGATGGAATTTATGGCG	AAAATTTGTGCGGCAGCTAC	50.0	45.0	58.0	57.9	1428 bp	0.5	59.4
ndhI_p5	GGGGATGGAATTTATGGCGT	ATTTTCTCGGCGGGCTAAAA	50.0	45.0	58.0	58.2	1770 bp	0.5	58.6