Hotspot

ndhF

Genome · 111401-113509 · 2109 bp

Back to Orthotrichum

Pi 0.454

MarkerSeek score 80.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: Genome
length_bp: 2109
variable_sites: 835
pi: 0.454
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CTGTTCACCTCCGGTTTCAA	CGCAATCAAGCAGGAAAAGG	50.0	50.0	58.0	58.0	2789 bp	0.5	59.6
ndhF_p2	CTGTTCACCTCCGGTTTCAA	AGTGGGAAGTCGGGAAGTTA	50.0	50.0	58.0	58.0	2667 bp	0.5	59.5
ndhF_p3	CTGTTCACCTCCGGTTTCAA	TAGTGGGAAGTCGGGAAGTT	50.0	50.0	58.0	58.0	2668 bp	0.5	59.5
ndhF_p4	CTGTTCACCTCCGGTTTCAA	AAGTCGGGAAGTTAGGGGAA	50.0	50.0	58.0	58.0	2661 bp	0.5	59.4
ndhF_p5	CTGTTCACCTCCGGTTTCAA	CGCGATCGTACCGTCATTAT	50.0	50.0	58.0	58.0	2633 bp	0.5	59.4