Hotspot

ndhA

Genome · 104109-105939 · 1831 bp

Back to Orthotrichum

Pi 0.441

MarkerSeek score 81.40

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: Genome
length_bp: 1831
variable_sites: 723
pi: 0.441
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ATCCGGACCAATGTTACGTG	GACCAATATATCGTGCCGCT	50.0	50.0	58.0	57.9	2508 bp	0.5	59.2
ndhA_p2	ATCCGGACCAATGTTACGTG	AACACATACTTCGCAGGCAA	50.0	45.0	58.0	58.1	2651 bp	0.5	59.0
ndhA_p3	ATCCGGACCAATGTTACGTG	ACACATACTTCGCAGGCAAT	50.0	45.0	58.0	57.9	2650 bp	0.5	58.9
ndhA_p4	GGCTTATCCGGACCAATGTT	GACCAATATATCGTGCCGCT	50.0	50.0	57.9	57.9	2513 bp	0.5	58.9
ndhA_p5	AAATTGGGGCTTATCCGGAC	GACCAATATATCGTGCCGCT	50.0	50.0	57.9	57.9	2520 bp	0.5	58.8