Hotspot

chlN

Genome · 96017-97426 · 1410 bp

Back to Orthotrichum

Pi 0.451

MarkerSeek score 77.10

Primer available yes

Label chlN

Feature Metadata

label_name: chlN
feature_type: gene
region: Genome
length_bp: 1410
variable_sites: 456
pi: 0.451
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.72
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlN_p1	GTTGCGGAGGCTATGTTGTA	AGCACACAAAGCTGTAAGGG	50.0	50.0	58.0	58.4	2276 bp	0.5	56.7
chlN_p2	GTTGCGGAGGCTATGTTGTA	GCACACAAAGCTGTAAGGGA	50.0	50.0	58.0	58.4	2275 bp	0.5	56.7
chlN_p3	GTTGCGGAGGCTATGTTGTA	CCCATAGCACACAAAGCTGT	50.0	50.0	58.0	58.5	2281 bp	0.5	56.2
chlN_p4	TGCTGCTTCAGTTCGAGAAA	AGCACACAAAGCTGTAAGGG	45.0	50.0	57.8	58.4	2080 bp	0.5	55.0
chlN_p5	TTGCTGCTTCAGTTCGAGAA	AGCACACAAAGCTGTAAGGG	45.0	50.0	57.8	58.4	2081 bp	0.5	55.0