Hotspot

rps19-rpl22

Genome · 2425-2441 · 17 bp

Back to Orthotrichum

Pi 0.118
MarkerSeek score 79.40
Primer available yes
Label rps19-rpl22

Feature Metadata

label_name
rps19-rpl22
feature_type
igs
region
Genome
length_bp
17
variable_sites
2
pi
0.118
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19-rpl22_p1 TCGAGGACATGCAAAAAGTGA TGTTTGGCTAAAGCTTTGGC 42.9 45.0 58.2 57.5 104 bp 1.0 68.1
rps19-rpl22_p2 GGAGAATTTGCACCTACACG TGTTTGGCTAAAGCTTTGGC 50.0 45.0 56.8 57.5 130 bp 1.0 67.6
rps19-rpl22_p3 AGGAGAATTTGCACCTACACG TGTTTGGCTAAAGCTTTGGC 47.6 45.0 58.3 57.5 131 bp 1.0 66.6
rps19-rpl22_p4 GGAGAATTTGCACCTACACGA TGTTTGGCTAAAGCTTTGGC 47.6 45.0 58.3 57.5 130 bp 1.0 66.6
rps19-rpl22_p5 CGAGGACATGCAAAAAGTGA TGTTTGGCTAAAGCTTTGGC 45.0 45.0 56.7 57.5 103 bp 1.0 66.6