Hotspot

ycf1

SSC · 107722-113274 · 5553 bp

Back to Orthosiphon

Pi 0.352

MarkerSeek score 75.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5553
variable_sites: 1715
pi: 0.352
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TAGCAGAGACCTGGGATAGC	CAATGGGGAATTTGCCGAAC	55.0	50.0	58.0	58.0	758 bp	0.5	59.9
ycf1_p2	ACGGATACTAAGATGCCCGA	CAATGGGGAATTTGCCGAAC	50.0	50.0	58.0	58.0	1101 bp	0.5	59.9
ycf1_p3	TAGCAGAGACCTGGGATAGC	AATGGGGAATTTGCCGAACT	55.0	45.0	58.0	58.1	757 bp	0.5	59.5
ycf1_p4	TAGCAGAGACCTGGGATAGC	ATGGGGAATTTGCCGAACTT	55.0	45.0	58.0	58.1	756 bp	0.5	59.5
ycf1_p5	ACGGATACTAAGATGCCCGA	ATGGGGAATTTGCCGAACTT	50.0	45.0	58.0	58.1	1099 bp	0.5	59.4