Hotspot

rpl32

SSC · 123628-123798 · 171 bp

Back to Orthosiphon

Pi 0.45

MarkerSeek score 76.90

Primer available yes

Label rpl32

Feature Metadata

label_name: rpl32
feature_type: gene
region: SSC
length_bp: 171
variable_sites: 72
pi: 0.45
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32_p1	GCTGGGGGAAATAAGACTCC	GCCCACATACGACGAAGATT	55.0	50.0	57.6	58.1	1019 bp	0.5	56.7
rpl32_p2	CACTAAGCGTTTTTGCGTGA	GCCCACATACGACGAAGATT	45.0	50.0	57.6	58.1	1154 bp	0.5	56.5
rpl32_p3	GCTGGGGGAAATAAGACTCC	AAAAGCCCACATACGACGAA	55.0	45.0	57.6	57.8	1023 bp	0.5	55.8
rpl32_p4	GCTGGGGGAAATAAGACTCC	TGGAAAAGCCCACATACGAC	55.0	50.0	57.6	58.2	1026 bp	0.5	55.7
rpl32_p5	TGCGTGAATTGATGGTGAGA	GCCCACATACGACGAAGATT	45.0	50.0	57.5	58.1	1141 bp	0.5	55.7