Hotspot

ndhI

SSC · 117454-117960 · 507 bp

Back to Orthosiphon

Pi 0.498

MarkerSeek score 81.50

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 507
variable_sites: 237
pi: 0.498
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	ACGGTTCTGTAATAGCGACG	CCCAACGGTCCAAAGATGAA	50.0	50.0	57.8	58.1	1825 bp	0.5	57.8
ndhI_p2	GTGACCTATGAGGAGCCGTA	CCCAACGGTCCAAAGATGAA	55.0	50.0	58.3	58.1	1864 bp	0.5	56.7
ndhI_p3	ACGGTTCTGTAATAGCGACG	AATCCCAATGAAAAGGCCGA	50.0	45.0	57.8	57.8	1659 bp	0.5	56.7
ndhI_p4	ACGGTTCTGTAATAGCGACG	ATCCCAATGAAAAGGCCGAA	50.0	45.0	57.8	57.8	1658 bp	0.5	56.7
ndhI_p5	ACGGTTCTGTAATAGCGACG	TCCCAATGAAAAGGCCGAAT	50.0	45.0	57.8	57.8	1657 bp	0.5	56.7