Hotspot

rpl32-ndhF

SSC · 123799-124276 · 478 bp

Back to Orthosiphon

Pi 0.397

MarkerSeek score 77.30

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 478
variable_sites: 155
pi: 0.397
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	AGAAAACGCTTTTAACGCCG	GCCCACATACGACGAAGATT	45.0	50.0	58.0	58.1	699 bp	0.5	59.3
rpl32-ndhF_p2	AGAAAACGCTTTTAACGCCG	AAAAGCCCACATACGACGAA	45.0	45.0	58.0	57.8	703 bp	0.5	58.4
rpl32-ndhF_p3	AGAAAACGCTTTTAACGCCG	TGGAAAAGCCCACATACGAC	45.0	50.0	58.0	58.2	706 bp	0.5	58.3
rpl32-ndhF_p4	AGAAAACGCTTTTAACGCCG	AAAGCCCACATACGACGAAG	45.0	50.0	58.0	58.3	702 bp	0.5	57.6
rpl32-ndhF_p5	AGAAAACGCTTTTAACGCCG	GAAAAGCCCACATACGACGA	45.0	50.0	58.0	58.3	704 bp	0.5	57.5