Hotspot

ndhI-ndhG

SSC · 117961-118322 · 362 bp

Back to Orthosiphon

Pi 0.503

MarkerSeek score 77.90

Primer available yes

Label ndhI-ndhG

Feature Metadata

label_name: ndhI-ndhG
feature_type: igs
region: SSC
length_bp: 362
variable_sites: 168
pi: 0.503
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhG_p1	CATGATTACCCTGTCCCACG	CCCAACGGTCCAAAGATGAA	55.0	50.0	58.0	58.1	1080 bp	0.5	58.9
ndhI-ndhG_p2	ACATCAGAACGTTTCCGAGG	CCCAACGGTCCAAAGATGAA	50.0	50.0	57.9	58.1	1013 bp	0.5	58.6
ndhI-ndhG_p3	TCAAATTGCTTTAGGCCGGT	CCCAACGGTCCAAAGATGAA	45.0	50.0	58.1	58.1	744 bp	0.5	58.6
ndhI-ndhG_p4	ACAAACAATACGAGCTGCCA	CCCAACGGTCCAAAGATGAA	45.0	50.0	58.1	58.1	1119 bp	0.5	58.4
ndhI-ndhG_p5	CTTGAACTCCGGTCGACAAA	CCCAACGGTCCAAAGATGAA	50.0	50.0	58.1	58.1	1134 bp	0.5	58.1