Hotspot

ycf1

SSC · 121522-126675 · 5154 bp

Back to Orostachys

Pi 0.0218

MarkerSeek score 50.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5154
variable_sites: 251
pi: 0.0218
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGCATGCCGCTTTTTGTTAA	AGACTTGCCGAAAAACTGGT	45.0	45.0	58.2	57.9	634-640 bp	0.5	64.3
ycf1_p2	GGCATGCCGCTTTTTGTTAA	ATTTCCCGAATGGTCCGAAG	45.0	50.0	58.2	58.0	721-727 bp	0.5	63.9
ycf1_p3	GGCATGCCGCTTTTTGTTAA	CCCGAATGGTCCGAAGATTT	45.0	50.0	58.2	58.0	717-723 bp	0.5	63.9
ycf1_p4	GGCATGCCGCTTTTTGTTAA	TCCGAAAAAGACTTGCCGAA	45.0	45.0	58.2	58.0	648 bp	0.4	54.5
ycf1_p5	GGCATGCCGCTTTTTGTTAA	AGACCAACCAAACAGCCTTT	45.0	45.0	58.2	57.8	480 bp	0.4	53.4