Hotspot

rpl32-trnL-UAG

SSC · 111648-112086 · 439 bp

Back to Orostachys

Pi 0.0402

MarkerSeek score 69.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 439
variable_sites: 50
pi: 0.0402
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGCAGCGTTAAAAGCCTTTT	TTCGAACCGAGATGCTCAAG	45.0	50.0	57.9	58.0	563-587 bp	1.0	90.2
rpl32-trnL-UAG_p2	GGCAGCGTTAAAAGCCTTTT	TTAGAAGAGAATGTGCCGCC	45.0	50.0	57.9	58.0	590-614 bp	1.0	89.9
rpl32-trnL-UAG_p3	GGCAGCGTTAAAAGCCTTTT	AAGCACTGCTTCCTAAGAGC	45.0	50.0	57.9	57.9	546-570 bp	1.0	89.6
rpl32-trnL-UAG_p4	AAAAGGAAGGGGTATTGGGC	TTCGAACCGAGATGCTCAAG	50.0	50.0	57.8	58.0	580-604 bp	1.0	89.4
rpl32-trnL-UAG_p5	AAAAGGAAGGGGTATTGGGC	TTAGAAGAGAATGTGCCGCC	50.0	50.0	57.8	58.0	607-631 bp	1.0	89.0