Hotspot

ndhF-rpl32

SSC · 110885-111488 · 604 bp

Back to Orostachys

Pi 0.0246

MarkerSeek score 63.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 604
variable_sites: 35
pi: 0.0246
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAAAGACCACACACGTCGAA	TTCGAACCGAGATGCTCAAG	45.0	50.0	58.0	58.0	1348-1395 bp	1.0	89.3
ndhF-rpl32_p2	AAAAGACCACACACGTCGAA	TTAGAAGAGAATGTGCCGCC	45.0	50.0	58.0	58.0	1375-1422 bp	1.0	89.1
ndhF-rpl32_p3	CAAGGGGTCGTGTAAACCAA	TTCGAACCGAGATGCTCAAG	50.0	50.0	58.0	58.0	1733-1780 bp	1.0	87.6
ndhF-rpl32_p4	CAAGGGGTCGTGTAAACCAA	TTAGAAGAGAATGTGCCGCC	50.0	50.0	58.0	58.0	1760-1807 bp	1.0	87.4
ndhF-rpl32_p5	CCAAAATCCCCGATACGGTT	TTCGAACCGAGATGCTCAAG	50.0	50.0	58.0	58.0	1785-1832 bp	1.0	87.2