Hotspot

rps3-rpl22

LSC · 82193-82300 · 108 bp

Back to Orostachys

Pi 0.0504

MarkerSeek score 62.00

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 108
variable_sites: 11
pi: 0.0504
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	CAAGTCGGAAACCAAGTGGA	TCCCGGGCATCTACCATTAT	50.0	50.0	58.0	58.0	751-764 bp	1.0	82.9
rps3-rpl22_p2	TGCTCGAATCGTTTGTAGGG	TCCCGGGCATCTACCATTAT	50.0	50.0	58.0	58.0	1287-1300 bp	1.0	82.8
rps3-rpl22_p3	CTCGGATCCATTCAACACGT	TCCCGGGCATCTACCATTAT	50.0	50.0	58.0	58.0	1255-1268 bp	1.0	82.8
rps3-rpl22_p4	CCCTACCTTCTCGGATCCAT	TCCCGGGCATCTACCATTAT	55.0	50.0	58.0	58.0	1264-1277 bp	1.0	82.7
rps3-rpl22_p5	CGACACCGGAAGCTATTCTC	TCCCGGGCATCTACCATTAT	55.0	50.0	58.2	58.0	886-899 bp	0.9	77.8