Hotspot

psbM-trnD-GUC

LSC · 29720-30723 · 1004 bp

Back to Orostachys

Pi 0.0189
MarkerSeek score 59.60
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1004
variable_sites
42
pi
0.0189
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGAATGAACAGTGCAGTAGC TCAATTGGTAAGAGCACCGC 45.0 50.0 55.8 58.6 1113-1149 bp 1.0 62.2
psbM-trnD-GUC_p2 AGAATGAACAGTGCAGTAGC AATTGGTAAGAGCACCGCC 45.0 52.6 55.8 58.1 1111-1147 bp 1.0 57.5
psbM-trnD-GUC_p3 AGAATGAACAGTGCAGTAGC GTTCAATTGGTAAGAGCACCG 45.0 47.6 55.8 57.5 1115-1151 bp 1.0 54.8
psbM-trnD-GUC_p4 AGAATGAACAGTGCAGTAGC CAATTGGTAAGAGCACCGCC 45.0 55.0 55.8 59.5 1112-1148 bp 1.0 54.2
psbM-trnD-GUC_p5 AGAATGAACAGTGCAGTAGC TTCAATTGGTAAGAGCACCG 45.0 45.0 55.8 56.1 1114-1150 bp 1.0 51.0