Hotspot

petN-psbM

LSC · 28573-29614 · 1042 bp

Back to Orostachys

Pi 0.0191

MarkerSeek score 59.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1042
variable_sites: 49
pi: 0.0191
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	GTTCCTACTGCCTTTTTACT	45.0	40.0	58.0	52.8	1157-1178 bp	1.0	46.2
petN-psbM_p2	TCTTGCTTGGGCTGCTTTAA	GTTCCTACTGCCTTTTTACTT	45.0	38.1	58.0	53.7	1157-1178 bp	1.0	46.2
petN-psbM_p3	AGTAAGTCTTGCTTGGGCTG	GTTCCTACTGCCTTTTTACT	50.0	40.0	57.8	52.8	1163-1184 bp	1.0	46.2
petN-psbM_p4	AGTAAGTCTTGCTTGGGCTG	GTTCCTACTGCCTTTTTACTT	50.0	38.1	57.8	53.7	1163-1184 bp	1.0	46.2
petN-psbM_p5	TCTTGCTTGGGCTGCTTTAA	GTTCCTACTGCCTTTTTACTTATCA	45.0	36.0	58.0	57.1	1157-1178 bp	1.0	46.2