Hotspot

trnC-GCA-petN

LSC · 27652-28482 · 831 bp

Back to Orostachys

Pi 0.0333

MarkerSeek score 65.70

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 831
variable_sites: 62
pi: 0.0333
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	879-902 bp	1.0	87.0
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	873-896 bp	1.0	85.5
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.4	58.0	897-920 bp	1.0	84.6
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTT	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.4	57.8	891-914 bp	1.0	83.2
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	TTAGAGTCCACTTCGTCCCC	50.0	55.0	58.1	58.4	935-958 bp	1.0	83.0