Hotspot

atpF-atpH

LSC · 13101-13482 · 382 bp

Back to Orostachys

Pi 0.0211

MarkerSeek score 59.30

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 382
variable_sites: 18
pi: 0.0211
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	CGAGGCGGAGGGAAAAATAA	50.0	50.0	57.9	58.0	547-570 bp	1.0	85.5
atpF-atpH_p2	GGATGACCAGTGACCCAAAT	CGAGGCGGAGGGAAAAATAA	50.0	50.0	57.5	58.0	526-549 bp	1.0	82.4
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	GCGGAGGGAAAAATAAGAGGT	50.0	47.6	57.9	58.0	543-566 bp	1.0	77.8
atpF-atpH_p4	TATTAAACCCGAAACTCCCGG	CGAGGCGGAGGGAAAAATAA	47.6	50.0	57.7	58.0	548-571 bp	1.0	76.3
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	GAGGCGGAGGGAAAAATAAGA	50.0	47.6	57.9	57.7	546-569 bp	1.0	75.5