Hotspot

rps16-trnQ-UUG

LSC · 5880-7146 · 1267 bp

Back to Orostachys

Pi 0.0269

MarkerSeek score 68.70

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1267
variable_sites: 79
pi: 0.0269
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1327-1364 bp	1.0	85.8
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1338-1375 bp	1.0	85.2
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	CTATGCGGAGGTTCGAATCC	45.0	55.0	58.1	58.2	1334-1371 bp	1.0	84.6
rps16-trnQ-UUG_p4	TCATGTCCTTCAAGTCGCAC	CTATGCGGAGGTTCGAATCC	50.0	55.0	58.2	58.2	1345-1382 bp	1.0	84.1
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	TATGCGGAGGTTCGAATCCT	45.0	50.0	58.1	58.6	1333-1370 bp	1.0	81.6