Hotspot

rps15-rps12

Genome · 64320-79413 · 15094 bp

Back to Orobanche

Pi 0.0334

MarkerSeek score 63.30

Primer available yes

Label rps15-rps12

Feature Metadata

label_name: rps15-rps12
feature_type: igs
region: Genome
length_bp: 15094
variable_sites: 688
pi: 0.0334
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-rps12_p1	CTCCCACAACCCCGTTTTAA	GGCTGTCAAGAACGTAGAGG	50.0	55.0	58.0	58.0	1141 bp	0.333333	53.2
rps15-rps12_p2	CTCCCACAACCCCGTTTTAA	GGGCTGTCAAGAACGTAGAG	50.0	55.0	58.0	58.0	1142 bp	0.333333	53.2
rps15-rps12_p3	ATTCGGGAATCTCCGGATCT	GGCTGTCAAGAACGTAGAGG	50.0	55.0	58.0	58.0	979 bp	0.333333	53.2
rps15-rps12_p4	ATTCGGGAATCTCCGGATCT	GGGCTGTCAAGAACGTAGAG	50.0	55.0	58.0	58.0	980 bp	0.333333	53.2
rps15-rps12_p5	CTCCCACAACCCCGTTTTAA	TGATTTCCTTCACGAGCGAG	50.0	50.0	58.0	58.0	1436 bp	0.333333	53.2