Hotspot

clpP-rpl36

Genome · 32728-34352 · 1625 bp

Back to Orobanche

Pi 0.0947

MarkerSeek score 79.50

Primer available yes

Label clpP-rpl36

Feature Metadata

label_name: clpP-rpl36
feature_type: igs
region: Genome
length_bp: 1625
variable_sites: 189
pi: 0.0947
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-rpl36_p1	AAAGGCCAGGGAGTGCTATA	ATCGGGAAGCTCGACTAGAA	50.0	50.0	58.1	57.9	2477-2484 bp	0.666667	73.5
clpP-rpl36_p2	GCCTTTGACCCTCGTATAGC	ATCGGGAAGCTCGACTAGAA	55.0	50.0	58.1	57.9	2385-2392 bp	0.666667	73.3
clpP-rpl36_p3	CCTAGTCCAATCGCAAGCTT	ATCGGGAAGCTCGACTAGAA	50.0	50.0	58.0	57.9	2362 bp	0.333333	52.7
clpP-rpl36_p4	GGTAGTGACCTCTGCCTTTG	ATCGGGAAGCTCGACTAGAA	55.0	50.0	57.9	57.9	2405 bp	0.333333	52.1
clpP-rpl36_p5	CCTAGTCCAATCGCAAGCTT	AGTCGACCTGGTCTACGAAT	50.0	50.0	58.0	57.9	2465 bp	0.333333	52.1