Hotspot

trnM-CAU-atpB

Genome · 22902-23535 · 634 bp

Back to Orobanche

Pi 0.071

MarkerSeek score 86.30

Primer available yes

Label trnM-CAU-atpB

Feature Metadata

label_name: trnM-CAU-atpB
feature_type: igs
region: Genome
length_bp: 634
variable_sites: 64
pi: 0.071
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 86.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpB_p1	GCGGGAGTCATTGGTTCAAA	GATGAAGCTATTGCGAAAG	50.0	42.1	58.8	52.1	673-701 bp	1.0	50.8
trnM-CAU-atpB_p2	GCGGGAGTCATTGGTTCAA	GATGAAGCTATTGCGAAAG	52.6	42.1	58.1	52.1	673-701 bp	1.0	50.8
trnM-CAU-atpB_p3	CTTTCATACGGCGGGAGTC	GATGAAGCTATTGCGAAAG	57.9	42.1	58.0	52.1	683-711 bp	1.0	50.6
trnM-CAU-atpB_p4	TATTGCTTTCATACGGCGGG	GATGAAGCTATTGCGAAAG	50.0	42.1	58.4	52.1	688-716 bp	1.0	50.5
trnM-CAU-atpB_p5	GTATTGCTTTCATACGGCGG	GATGAAGCTATTGCGAAAG	50.0	42.1	57.3	52.1	689-717 bp	1.0	50.5