Hotspot

trnfM-CAU-rps14

Genome · 17085-17232 · 148 bp

Back to Orobanche

Pi 0.119

MarkerSeek score 81.40

Primer available yes

Label trnfM-CAU-rps14

Feature Metadata

label_name: trnfM-CAU-rps14
feature_type: igs
region: Genome
length_bp: 148
variable_sites: 24
pi: 0.119
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnfM-CAU-rps14_p1	AAGGTTATGAGCCTTGCGAG	GCATGTTTGTTACCAGGAGC	50.0	50.0	58.0	57.4	220-230 bp	1.0	86.7
trnfM-CAU-rps14_p2	GATGTTGCGGAGACAGGATT	GCATGTTTGTTACCAGGAGC	50.0	50.0	58.0	57.4	255-265 bp	1.0	85.8
trnfM-CAU-rps14_p3	CCAAACTACTCTACTCCGCG	GCATGTTTGTTACCAGGAGC	55.0	50.0	57.8	57.4	197-207 bp	1.0	85.4
trnfM-CAU-rps14_p4	TGCGGAGACAGGATTTGAAC	GCATGTTTGTTACCAGGAGC	50.0	50.0	58.2	57.4	250-260 bp	1.0	84.7
trnfM-CAU-rps14_p5	AAAGATGTTGCGGAGACAGG	GCATGTTTGTTACCAGGAGC	50.0	50.0	58.2	57.4	258-268 bp	1.0	84.4