Hotspot

trnS-UGA-trnG-UCC

Genome · 16055-16762 · 708 bp

Back to Orobanche

Pi 0.0736

MarkerSeek score 83.10

Primer available yes

Label trnS-UGA-trnG-UCC

Feature Metadata

label_name: trnS-UGA-trnG-UCC
feature_type: igs
region: Genome
length_bp: 708
variable_sites: 76
pi: 0.0736
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-trnG-UCC_p1	AACCTCTCAGCCATCTCTCC	TGGCAAAGAGAAATTTTACCA	55.0	33.3	58.5	53.9	753-788 bp	1.0	49.4
trnS-UGA-trnG-UCC_p2	AACCTCTCAGCCATCTCTCC	TTGGCAAAGAGAAATTTTACCA	55.0	31.8	58.5	54.7	754-789 bp	1.0	49.4
trnS-UGA-trnG-UCC_p3	AACCTCTCAGCCATCTCTCC	TGGCAAAGAGAAATTTTACCAT	55.0	31.8	58.5	54.5	753-788 bp	1.0	49.4
trnS-UGA-trnG-UCC_p4	AACCTCTCAGCCATCTCTCC	TGGCAAAGAGAAATTTTACCATT	55.0	30.4	58.5	55.3	753-788 bp	1.0	49.4
trnS-UGA-trnG-UCC_p5	AACCTCTCAGCCATCTCTCC	TTGGCAAAGAGAAATTTTACCAT	55.0	30.4	58.5	55.3	754-789 bp	1.0	49.4