Hotspot

rps16-trnQ-UUG

Genome · 3827-4694 · 868 bp

Back to Orobanche

Pi 0.0654

MarkerSeek score 86.40

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: Genome
length_bp: 868
variable_sites: 82
pi: 0.0654
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 86.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CGTTGCTTTCTACCACATCG	TAAGGCACCGAGTTTTGGTC	50.0	50.0	57.2	58.1	963-991 bp	1.0	82.8
rps16-trnQ-UUG_p2	CGTTGCTTTCTACCACATCG	GTTTTGGTCTCGCTAGTCGA	50.0	50.0	57.2	57.7	952-980 bp	1.0	81.1
rps16-trnQ-UUG_p3	CGTTGCTTTCTACCACATCG	AGTTTTGGTCTCGCTAGTCG	50.0	50.0	57.2	57.7	953-981 bp	1.0	81.0
rps16-trnQ-UUG_p4	CGTTGCTTTCTACCACATCG	CGAGTTTTGGTCTCGCTAGT	50.0	50.0	57.2	57.7	955-983 bp	1.0	81.0
rps16-trnQ-UUG_p5	CGTTGCTTTCTACCACATCG	AAATTAAAATGGGGCGTGGC	50.0	45.0	57.2	57.6	990-1018 bp	1.0	80.3