Hotspot

rpl14-rps8

IRa · 162807-163634 · 828 bp

Back to Ormosia

Pi 0.038

MarkerSeek score 81.70

Primer available yes

Label rpl14-rps8

Feature Metadata

label_name: rpl14-rps8
feature_type: igs
region: IRa
length_bp: 828
variable_sites: 73
pi: 0.038
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rps8_p1	GAACTCGAATTTTTGGCGCA	TTATGTCAGCGATGGTGTCC	45.0	50.0	58.0	57.7	676-1013 bp	1.0	87.7
rpl14-rps8_p2	GTTTCATTAGCACCCGAGGT	TTATGTCAGCGATGGTGTCC	50.0	50.0	57.9	57.7	617-954 bp	1.0	87.7
rpl14-rps8_p3	GAACTCGAATTTTTGGCGCA	GTCAGCGATGGTGTCCTTAC	45.0	55.0	58.0	58.4	672-1009 bp	1.0	87.3
rpl14-rps8_p4	GTTTCATTAGCACCCGAGGT	GTCAGCGATGGTGTCCTTAC	50.0	55.0	57.9	58.4	613-950 bp	1.0	87.3
rpl14-rps8_p5	GAACTCGAATTTTTGGCGCA	TATGTCAGCGATGGTGTCCT	45.0	50.0	58.0	58.5	675-1012 bp	1.0	86.1