Hotspot

rpl32-trnL-UAG

SSC · 117752-118797 · 1046 bp

Back to Ormosia

Pi 0.0862

MarkerSeek score 76.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1046
variable_sites: 107
pi: 0.0862
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.47
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGGATATTGGACAGCATTGA	TCCTAAGAGCAGCGTGTCTA	47.6	50.0	57.8	57.9	601-1165 bp	1.0	81.2
rpl32-trnL-UAG_p2	AGGGGATATTGGACAGCATTG	TCCTAAGAGCAGCGTGTCTA	47.6	50.0	57.8	57.9	602-1166 bp	1.0	81.1
rpl32-trnL-UAG_p3	GGGGATATTGGACAGCATTGA	AGCAGCGTGTCTACCAATTT	47.6	45.0	57.8	57.8	594-1158 bp	1.0	80.7
rpl32-trnL-UAG_p4	AGGGGATATTGGACAGCATTG	AGCAGCGTGTCTACCAATTT	47.6	45.0	57.8	57.8	595-1159 bp	1.0	80.6
rpl32-trnL-UAG_p5	GGGGATATTGGACAGCATTGA	CTAAGAGCAGCGTGTCTACC	47.6	55.0	57.8	57.8	599-1163 bp	1.0	80.6