Hotspot

rps8-rpl14

IRb · 84440-85267 · 828 bp

Back to Ormosia

Pi 0.0298

MarkerSeek score 76.50

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: IRb
length_bp: 828
variable_sites: 63
pi: 0.0298
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	TTATGTCAGCGATGGTGTCC	GAACTCGAATTTTTGGCGCA	50.0	45.0	57.7	58.0	676-1013 bp	1.0	86.8
rps8-rpl14_p2	TTATGTCAGCGATGGTGTCC	GTTTCATTAGCACCCGAGGT	50.0	50.0	57.7	57.9	617-954 bp	1.0	86.7
rps8-rpl14_p3	GTCAGCGATGGTGTCCTTAC	GAACTCGAATTTTTGGCGCA	55.0	45.0	58.4	58.0	672-1009 bp	1.0	86.4
rps8-rpl14_p4	GTCAGCGATGGTGTCCTTAC	GTTTCATTAGCACCCGAGGT	55.0	50.0	58.4	57.9	613-950 bp	1.0	86.3
rps8-rpl14_p5	TATGTCAGCGATGGTGTCCT	GAACTCGAATTTTTGGCGCA	50.0	45.0	58.5	58.0	675-1012 bp	1.0	85.2