Hotspot

rps11-rpl36

IRb · 82884-83487 · 604 bp

Back to Ormosia

Pi 0.0286

MarkerSeek score 71.30

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: IRb
length_bp: 604
variable_sites: 44
pi: 0.0286
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	CGTCCATTTTTACGCGAACC	CGTAGGCAGGGACGAATTAT	50.0	50.0	58.1	57.2	662-739 bp	1.0	81.5
rps11-rpl36_p2	CGTCCATTTTTACGCGAACC	AAGGGCCTCTGTTCGTAAAA	50.0	45.0	58.1	57.1	705-782 bp	1.0	80.3
rps11-rpl36_p3	TTACGCGAACCAATACGTCC	CGTAGGCAGGGACGAATTAT	50.0	50.0	58.4	57.2	677-754 bp	1.0	79.5
rps11-rpl36_p4	CGTCCATTTTTACGCGAACC	AAATAAGGGCCTCTGTTCGT	50.0	45.0	58.1	56.8	709-786 bp	1.0	78.5
rps11-rpl36_p5	CGTCCATTTTTACGCGAACC	AGGGCCTCTGTTCGTAAAAT	50.0	45.0	58.1	56.8	704-781 bp	1.0	78.4