Hotspot

psbK-trnQ-UUG

LSC · 57262-58238 · 977 bp

Back to Ormosia

Pi 0.075

MarkerSeek score 79.20

Primer available yes

Label psbK-trnQ-UUG

Feature Metadata

label_name: psbK-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 977
variable_sites: 166
pi: 0.075
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-trnQ-UUG_p1	AGGGCAGAGTGAATACAAATACA	GACCAAAACCCGTTGCCTTA	39.1	50.0	57.6	58.7	1045-1180 bp	1.0	59.1
psbK-trnQ-UUG_p2	AAAGGGCAGAGTGAATACAA	GACCAAAACCCGTTGCCTTA	40.0	50.0	54.5	58.7	1047-1182 bp	1.0	58.9
psbK-trnQ-UUG_p3	AAGGGCAGAGTGAATACAAA	GACCAAAACCCGTTGCCTTA	40.0	50.0	54.5	58.7	1046-1181 bp	1.0	58.9
psbK-trnQ-UUG_p4	AAAGGGCAGAGTGAATACAAA	GACCAAAACCCGTTGCCTTA	38.1	50.0	55.4	58.7	1047-1182 bp	1.0	57.7
psbK-trnQ-UUG_p5	AGGGCAGAGTGAATACAAATACA	GAATAGCGGGACCAAAACCC	39.1	55.0	57.6	58.9	1054-1189 bp	1.0	57.3