Hotspot

trnD-GUC-trnY-GUA

LSC · 40565-41027 · 463 bp

Back to Ormosia

Pi 0.039

MarkerSeek score 78.90

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 463
variable_sites: 42
pi: 0.039
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	CGGTGCTCTGACCAATTGAA	ATTCTTCTTGGGTCGATGCC	50.0	50.0	58.5	58.0	566-586 bp	1.0	85.8
trnD-GUC-trnY-GUA_p2	CGGTGCTCTGACCAATTGAA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.5	57.8	513-533 bp	1.0	84.7
trnD-GUC-trnY-GUA_p3	CGGTGCTCTGACCAATTGAA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.5	57.8	512-532 bp	1.0	84.7
trnD-GUC-trnY-GUA_p4	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.5	58.4	534-554 bp	1.0	83.5
trnD-GUC-trnY-GUA_p5	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.5	57.6	533-553 bp	1.0	83.4