Hotspot

trnC-GCA-petN

LSC · 37421-38620 · 1200 bp

Back to Ormosia

Pi 0.0473

MarkerSeek score 77.50

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 1200
variable_sites: 139
pi: 0.0473
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTTGTATCGTTTTGGCGGC	TTAAAGCAGCCCAAGCAAGA	45.0	45.0	57.9	58.0	685-1324 bp	1.0	91.5
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	629-1268 bp	1.0	91.3
trnC-GCA-petN_p3	TTTTGTATCGTTTTGGCGGC	CAGCCCAAGCAAGACTTACT	45.0	50.0	57.9	57.8	679-1318 bp	1.0	90.0
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	623-1262 bp	1.0	89.8
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.4	58.0	647-1286 bp	1.0	89.1