Hotspot

atpH-atpI

LSC · 22626-23958 · 1333 bp

Back to Ormosia

Pi 0.0241

MarkerSeek score 66.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1333
variable_sites: 63
pi: 0.0241
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.60
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	GCTGCGGCTTATATAGGTGA	45.0	50.0	58.0	57.3	803-1407 bp	1.0	82.9
atpH-atpI_p2	CGCAATCCCTTCTACAGCTT	GCTGCGGCTTATATAGGTGA	50.0	50.0	58.0	57.3	891-1495 bp	1.0	82.5
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	ACAAGCGGTATTCAAGCTCT	45.0	45.0	58.0	57.2	839-1443 bp	1.0	82.5
atpH-atpI_p4	CGCAATCCCTTCTACAGCTT	ACAAGCGGTATTCAAGCTCT	50.0	45.0	58.0	57.2	927-1531 bp	1.0	82.2
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	GCTGCGGCTTATATAGGTGA	45.0	50.0	58.2	57.3	804-1408 bp	1.0	81.7