Hotspot

rbcL

LSC · 55169-56596 · 1428 bp

Back to Orinus

Pi 0.0035
MarkerSeek score 60.80
Primer available yes
Label rbcL

Feature Metadata

label_name
rbcL
feature_type
gene
region
LSC
length_bp
1428
variable_sites
5
pi
0.0035
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL_p1 CATATCGAGCAGACCCTGTC ACTGCATTTCGTAACTCATT 55.0 35.0 57.6 53.2 1578 bp 1.0 40.3
rbcL_p2 GACCCTGTCGTTGTGAGAAT ACTGCATTTCGTAACTCATT 50.0 35.0 57.5 53.2 1567 bp 1.0 40.3
rbcL_p3 CATATCGAGCAGACCCTGTC ACTGCATTTCGTAACTCATTT 55.0 33.3 57.6 54.1 1578 bp 1.0 40.3
rbcL_p4 GACCCTGTCGTTGTGAGAAT ACTGCATTTCGTAACTCATTT 50.0 33.3 57.5 54.1 1567 bp 1.0 40.3
rbcL_p5 AGACCCTGTCGTTGTGAGAA ACTGCATTTCGTAACTCATT 50.0 35.0 58.6 53.2 1568 bp 1.0 40.3