Hotspot

petA

LSC · 59788-60750 · 963 bp

Back to Orinus

Pi 0
MarkerSeek score 25.40
Primer available yes
Label petA

Feature Metadata

label_name
petA
feature_type
gene
region
LSC
length_bp
963
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
25.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA_p1 CCGTCTATCTCCTTCGCTTG TTATTGTTCTCGCCTCTGCC 55.0 50.0 57.9 58.0 1342 bp 1.0 78.9
petA_p2 CCGTCTATCTCCTTCGCTTG TCCAAGAGACAGACCCGAAT 55.0 50.0 57.9 58.1 1523 bp 1.0 78.9
petA_p3 CCGTCTATCTCCTTCGCTTG TTTGCCCCCACCATACTTTC 55.0 50.0 57.9 58.1 1590 bp 1.0 78.8
petA_p4 CCGTCTATCTCCTTCGCTTG CCTCTGTTTGTCCACTACCG 55.0 55.0 57.9 57.9 1566 bp 1.0 78.7
petA_p5 CCGTCTATCTCCTTCGCTTG GCTCGAACCTAAGCAAAGGA 55.0 50.0 57.9 57.9 1545 bp 1.0 78.6