Hotspot

ndhF

SSC · 100955-103174 · 2220 bp

Back to Orinus

Pi 0.0036
MarkerSeek score 46.90
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2220
variable_sites
8
pi
0.0036
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 TGCATTTTGATGAGCCTCGT AGCAACTAAACCCATTCCGA 45.0 45.0 58.2 57.1 2466 bp 1.0 72.0
ndhF_p2 TGCATTTTGATGAGCCTCGT AAGCAACTAAACCCATTCCGA 45.0 42.9 58.2 57.9 2467 bp 1.0 69.7
ndhF_p3 TGCATTTTGATGAGCCTCGT AGCAACTAAACCCATTCCGAA 45.0 42.9 58.2 57.9 2466 bp 1.0 69.7
ndhF_p4 GTTTGCTGGCTTATTTGGCA AGCAACTAAACCCATTCCGA 45.0 45.0 57.5 57.1 2604 bp 1.0 69.7
ndhF_p5 ACGGAGACTTGCTTCACATT AGCAACTAAACCCATTCCGA 45.0 45.0 57.4 57.1 2690 bp 1.0 68.9