Hotspot

ccsA

SSC · 104946-105914 · 969 bp

Back to Orinus

Pi 0.0031
MarkerSeek score 50.60
Primer available yes
Label ccsA

Feature Metadata

label_name
ccsA
feature_type
gene
region
SSC
length_bp
969
variable_sites
3
pi
0.0031
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA_p1 GCGGCATTCTCGAAAAAGAA GGGGTTCCCAAATCAAAAA 45.0 42.1 57.4 53.6 1114 bp 1.0 40.2
ccsA_p2 GCGGCATTCTCGAAAAAGAAT GGGGTTCCCAAATCAAAAA 42.9 42.1 57.9 53.6 1114 bp 1.0 40.2
ccsA_p3 GGCGGCATTCTCGAAAAAGA GGGGTTCCCAAATCAAAAA 50.0 42.1 59.2 53.6 1115 bp 1.0 40.2
ccsA_p4 GGCGGCATTCTCGAAAAAG GGGGTTCCCAAATCAAAAA 52.6 42.1 57.7 53.6 1115 bp 1.0 40.2
ccsA_p5 TGGCGGCATTCTCGAAAAA GGGGTTCCCAAATCAAAAA 47.4 42.1 58.4 53.6 1116 bp 1.0 40.2