Hotspot

atpB

LSC · 52880-54376 · 1497 bp

Back to Orinus

Pi 0.002
MarkerSeek score 46.00
Primer available yes
Label atpB

Feature Metadata

label_name
atpB
feature_type
gene
region
LSC
length_bp
1497
variable_sites
3
pi
0.002
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB_p1 AACCGCTGTTAACCATTGGT GAATGGGATTCGCTTAGGCA 45.0 50.0 58.0 58.0 1862-1863 bp 1.0 79.9
atpB_p2 AACCGCTGTTAACCATTGGT TGAATGGGATTCGCTTAGGC 45.0 50.0 58.0 58.0 1863-1864 bp 1.0 79.9
atpB_p3 CGTACCCTCAGCTCTACTCA GAATGGGATTCGCTTAGGCA 55.0 50.0 58.0 58.0 2006-2007 bp 1.0 79.6
atpB_p4 CGTACCCTCAGCTCTACTCA TGAATGGGATTCGCTTAGGC 55.0 50.0 58.0 58.0 2007-2008 bp 1.0 79.6
atpB_p5 TTCAAGTGCCTGTTGAGCTT GAATGGGATTCGCTTAGGCA 45.0 50.0 57.9 58.0 1970-1971 bp 1.0 79.5