Hotspot

ccsA-ndhD

SSC · 105915-106104 · 190 bp

Back to Orinus

Pi 0.037
MarkerSeek score 66.70
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
190
variable_sites
7
pi
0.037
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TGCATTTGTAGCTTCGATAGGA CTCTCGCTATCCGTTGACAG 40.9 55.0 57.6 57.9 358-359 bp 1.0 61.7
ccsA-ndhD_p2 TGCATTTGTAGCTTCGATAGGA TTGACAGGGTAGAGGCTCTC 40.9 55.0 57.6 58.1 345-346 bp 1.0 61.5
ccsA-ndhD_p3 TGCATTTGTAGCTTCGATAGGA GACAGGGTAGAGGCTCTCTT 40.9 55.0 57.6 57.8 343-344 bp 1.0 61.4
ccsA-ndhD_p4 TGCATTTGTAGCTTCGATAGGA TCTCTCGCTATCCGTTGACA 40.9 50.0 57.6 58.3 359-360 bp 1.0 60.5
ccsA-ndhD_p5 TTCTGCATTTGTAGCTTCGA CTCTCGCTATCCGTTGACAG 40.0 55.0 55.4 57.9 361-362 bp 1.0 60.1