Hotspot

rpl32-trnL-UAG

SSC · 104264-104788 · 525 bp

Back to Orinus

Pi 0.0154
MarkerSeek score 66.70
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
525
variable_sites
8
pi
0.0154
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGTAACGAGCATCCAAAACC TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.1 57.9 599-603 bp 1.0 73.3
rpl32-trnL-UAG_p2 GGTAACGAGCATCCAAAACC AGCAGCGTGTCTACCAATTT 50.0 45.0 57.1 57.8 592-596 bp 1.0 72.8
rpl32-trnL-UAG_p3 GGTAACGAGCATCCAAAACC GCTTGAGCACTGCTTCCTAA 50.0 50.0 57.1 58.2 613-617 bp 1.0 72.8
rpl32-trnL-UAG_p4 GGTAACGAGCATCCAAAACC CCTAAGAGCAGCGTGTCTAC 50.0 55.0 57.1 57.8 598-602 bp 1.0 72.7
rpl32-trnL-UAG_p5 GGTAACGAGCATCCAAAACC CTAAGAGCAGCGTGTCTACC 50.0 55.0 57.1 57.8 597-601 bp 1.0 72.7