Hotspot

petA-psbJ

LSC · 60751-61577 · 827 bp

Back to Orinus

Pi 0.0109
MarkerSeek score 65.10
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
827
variable_sites
9
pi
0.0109
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 CGTCCAAGGCCTTTTGTTCT ACGGTTCGTATTCTGGATTGG 50.0 47.6 58.7 58.1 971-1050 bp 1.0 66.6
petA-psbJ_p2 GATCCATTACGCGTCCAAGG ACGGTTCGTATTCTGGATTGG 55.0 47.6 58.8 58.1 982-1061 bp 1.0 65.7
petA-psbJ_p3 CGTCCAAGGCCTTTTGTTCT GGTTCGTATTCTGGATTGGGT 50.0 47.6 58.7 57.7 969-1048 bp 1.0 65.1
petA-psbJ_p4 CGTCCAAGGCCTTTTGTTCT GCTGATAGGTACTGTAACCGG 50.0 52.4 58.7 57.6 1027-1106 bp 1.0 64.4
petA-psbJ_p5 CGTCCAAGGCCTTTTGTTCT GGCTGATAGGTACTGTAACCG 50.0 52.4 58.7 57.6 1028-1107 bp 1.0 64.4