Hotspot

atpB-rbcL

LSC · 54377-55168 · 792 bp

Back to Orinus

Pi 0.00506
MarkerSeek score 61.80
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
792
variable_sites
4
pi
0.00506
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 TTTGATCAATACGCCCTGCA GCTTGAAATCCAACACCTGC 45.0 50.0 57.9 57.9 912-913 bp 1.0 78.9
atpB-rbcL_p2 TTTGATCAATACGCCCTGCA TTCGTATTCCGGGGTGTAGT 45.0 50.0 57.9 58.1 958-959 bp 1.0 78.3
atpB-rbcL_p3 TATCCAGCACGGGTCCAATA GCTTGAAATCCAACACCTGC 50.0 50.0 58.2 57.9 933-934 bp 1.0 78.2
atpB-rbcL_p4 CCAGCACGGGTCCAATAATT GCTTGAAATCCAACACCTGC 50.0 50.0 58.2 57.9 930-931 bp 1.0 78.0
atpB-rbcL_p5 TATCCAGCACGGGTCCAATA TTCGTATTCCGGGGTGTAGT 50.0 50.0 58.2 58.1 979-980 bp 1.0 77.6