Hotspot

ndhC-trnV-UAC

LSC · 50487-51412 · 926 bp

Back to Orinus

Pi 0.00433
MarkerSeek score 61.60
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
926
variable_sites
4
pi
0.00433
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCTGGTCCTTCACTACTCGG GGTTCGAATCCGTATAGCCC 55.0 55.0 58.2 57.9 1062-1064 bp 1.0 78.6
ndhC-trnV-UAC_p2 TCTGGTCCTTCACTACTCGG AGCCCAATGCAGAAAAATGC 55.0 45.0 58.2 57.9 1155-1157 bp 1.0 78.3
ndhC-trnV-UAC_p3 CACTACTCGGGGCTAAAAGC GGTTCGAATCCGTATAGCCC 55.0 55.0 58.3 57.9 1052-1054 bp 1.0 77.1
ndhC-trnV-UAC_p4 CACTACTCGGGGCTAAAAGC AGCCCAATGCAGAAAAATGC 55.0 45.0 58.3 57.9 1145-1147 bp 1.0 76.9
ndhC-trnV-UAC_p5 TCTGGTCCTTCACTACTCGG CGGTTCGAATCCGTATAGCC 55.0 55.0 58.2 58.3 1063-1065 bp 1.0 76.8