Hotspot

psbM-petN

LSC · 17162-17969 · 808 bp

Pi 0.00499

MarkerSeek score 61.40

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 808
variable_sites: 4
pi: 0.00499
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	AAAACAGCCAGCCAAAATGA	TAGTAAGTCTCGCTTGGGCT	40.0	50.0	56.7	58.2	910-915 bp	1.0	69.3
psbM-petN_p2	AAAACAGCCAGCCAAAATGA	GGGGGAGAAGTGGACTCTAG	40.0	60.0	56.7	58.2	847-852 bp	1.0	68.8
psbM-petN_p3	AAAACAGCCAGCCAAAATGA	AGTAGTCTGGGGGAGAAGTG	40.0	55.0	56.7	57.8	855-860 bp	1.0	68.6
psbM-petN_p4	AAACAGCCAGCCAAAATGAT	TAGTAAGTCTCGCTTGGGCT	40.0	50.0	56.5	58.2	909-914 bp	1.0	67.4
psbM-petN_p5	AACAGCCAGCCAAAATGATT	TAGTAAGTCTCGCTTGGGCT	40.0	50.0	56.5	58.2	908-913 bp	1.0	67.4